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Antitumor activities of nonviable, aqueous ether-extracted bacterial adjuvant preparations (BAP) of Mycobacterium bovis, Propionibacterium acnes, Brucella abortus, Bordetella bronchiseptica, Salmonella typhi and Candida albicans against Sarcoma180 ascites tumor were compared in vivo. The protective effects of BAP against Sarcoma 180 tumor cells in ICRJCL mice were assessed by the time and the route of injection and the repeated doses. Followings were the results obtained : 1. When a 2,000 ㎍ of the experimental BAP was administered intraperitoneally as early as one day before peritoneal challenge with 10^4 tumor cells although the occurrance of ascites tumors was not affected, a statistically significant prolongation of the mean survival time was observed in the mice injected with BAP from B. abortus (37.3±8.5 day), B. bronchiseptica (36.6±10.6 day)±, C. albicans (34.8±13.5 days) and S. typhi (33.8±7.5 days) in comparison with untreated control (26.0 3.3 days). BAP both from M. bovis (27.2±19.7 days) and P. acnes (29.8±2.8 days), however, failed to induce such a protective effect. 2. Although a single intraperitoneal injection of a 2,000 ㎍ of BAP of B. abortus (24.1±1.4 days), B. bronchiseptica ((24.6±4.1 days) and C. albicans (25.6±3.7 days) as late as 7 days after the inoculation of 10^4 tumor cells was not effective, a booster intradermal injection 7 days after the first injection with a 1,000 ㎍ of BAP of B. abortus (28.5±2.4 days) and C. albicans (30.7±2.3 days) significantly prolonged the mean survival days. 3. Three consecutive intradermal injections of a 1,000 ㎍ of BAP of B. abortus (23.7±4.5 days), B. bronchiseptica (25.2±4.9 days) and C. albicans (22.4±4.0 days) given 7, 14 and 21 days after inoculation of 10^4 tumor cells proved ineffective. These results indicated that the BAP prepared from B. abortus and C. albicans provided better protection than the others against Sarcoma180 ascites tumors in ICRJCL mice and the extent of protection appeared closely associated with time and route of injection and repeated doses of BAP.
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